G protein-coupled receptors (GPCRs) are key players of cellular signal transduction. This collection aims to highlight new experimental techniques available for studies of signal transduction and establish best practices for the practical implementation of methods used in GPCR research. We also intend to define community-accepted standards for reporting signaling properties. Therefore, we welcome different submission types, including Original Research, Reviews, Mini Reviews, Systematic Reviews, Methods, Hypothesis & Theory, Perspective, etc.
With this collection, the Topic Editors aim to illustrate the most advanced biophysical and biological methodologies that can be useful in the elucidation of GPCRs structure and signal transduction mechanisms, create best practice guidelines for these methodologies, and collect community-accepted standards for reporting of signaling properties, such as biased agonism, intracellular signaling, etc.
The Editors are interested in works covering the following areas:
- The latest advances in imaging and spectroscopy techniques and optical biosensors to study the mechanisms of second effectors recruitment and signal transduction
- Biophysical techniques to study GPCR structure (NMR, cryo-EM, X-ray crystallography, Free electron lasers, In-cell crystallization etc.)
- Development of fluorescent and luminescent probes to study cell signaling mechanisms
- Novel biochemical techniques for studies of signal transduction (e.g., cell-free expression of membrane proteins, new ligand binding assays, thermostability assays)
- Label-free technologies
- Biased agonism-related research
This collection has been generated as part of the ERNEST COST Action
collaboration.Please also see the collection "Advances in Computational and Chemical Methods to study GPCRs Signal Transduction", which focuses on bioinformatic and chemical approaches for studying GPCRs.